March 3, 2026

Utility of flow cytometric PAX5 protein detection for the diagnosis of B-cell acute lymphoblastic leukemia

Key Points

nPAX5 achieved 100% agreement with traditional immunohistochemistry methods, confirming its reliability.
The diagnostic performance of nPAX5 shows an AUC of 0.955 with a sensitivity of 91.06%, making it highly effective.
Utilizing flow cytometry allows for rapid B-lineage assignment in acute leukemia diagnostics.
These findings support the integration of nPAX5 as a standard marker in clinical practice for diagnosing B-cell malignancies.

Abstract

Flow cytometric nuclear PAX5 (nPAX5) demonstrated 100 % agreement with IHC and exhibited excellent diagnostic accuracy (AUC 0.955, 91.06 % sensitivity). These compelling results validate nPAX5 as a reliable and powerful FCM marker, supporting its immediate adoption for rapid and efficient B-lineage assignment in acute leukemia diagnostics.

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Utility of flow cytometric PAX5 protein detection for the diagnosis of B-cell acute lymphoblastic leukemia

Key Points

Abstract

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