AbstractThe value of LC50 of the commercially available immunosuppressive drug methotrexate (MTX) was determined using the larvae of Drosophila melanogaster. For this purpose, third instar larvae were exposed to different concentrations of MTX (75, 100, 150, 200 and 250 ppm). Probit analysis was done on the dose-response data in order to find LC50. The results indicated that after 24 hours of acute exposure, larvae subjected to 164.9 ppm MTX showed 50% mortality. Hence the LC50 value was found to be 164.9 ppm for MTX in third instar larvae of Drosophila melanogaster.
Patel et al. (Wed,) studied this question.