This study investigates the foliar secondary metabolite profiles of four Mitragyna species naturally occurring in Thailand: M. diversifolia, M. hirsuta, M. rotundifolia, and M. speciosa (kratom). Using untargeted gas chromatography-mass spectrometry (GC-MS), 409 secondary volatile metabolites were annotated across the four species. M. diversifolia exhibited the highest number of detected volatile metabolites (87 ± 7), followed by M. hirsuta (75 ± 7), M. rotundifolia (74 ± 15), and M. speciosa (49 ± 11). Despite its lower overall metabolite count, M. speciosa had the highest number of unique compounds distinguishing it from the other species. Ten key volatile metabolites, including mitragynine, speciogynine, speciociliatine, paynantheine, isopaynantheine, and ajmalicine, were identified as major discriminants by Partial Least Squares Discriminant Analysis (PLS-DA). Leaf traits such as chlorophyll content and leaf pH showed positive correlations with metabolite abundance (r = 0.49 and 0.47; p-value r = -0.51; p-value T max (28.04%), vapor pressure deficit, drought, and wind (13.56%) significantly influenced metabolite composition (p-value Mitragyna species and provide a foundation for further research and exploration of these species in various scientific and medicinal contexts.
Andriyas et al. (Wed,) studied this question.