Proteolytic extracellular enzymes of Pseudomonas fragi drive spoilage metabolism in grouper (Epinephelus lanceolatus) during cold storage: insights into degradation pathways

Key Points

• Spoilage metabolism is primarily driven by the proteolytic enzymes from Pseudomonas fragi, which contribute to food degradation.
• The study measures key degradation pathways, revealing significant enzyme activity in grouper during cold storage.
• Observational analysis examined proteolytic activity over time under cold storage conditions to identify spoilage mechanisms.
• Findings highlight the need for improved storage methods to extend the shelf life of grouper fish, addressing spoilage concerns.