Combinatorial optimization of cellular regulation and fermentation for high-efficiency expression of cephalosporin C acylase in Escherichia coli

Key Points

• High-efficiency expression of cephalosporin C acylase was achieved, indicating substantial improvements in fermentation methods.
• The optimization process focused on combinatorial methods, integrating cellular regulation and production techniques effectively.
• Utilizing Escherichia coli as a host organism, the study explored various fermentation conditions and genetic modifications.
• Results support further exploration of these strategies for industrial-scale bioproduction, highlighting their potential in biotechnology.