What question did this study set out to answer?

To isolate and evaluate the antimicrobial activity of lupane-type triterpenoids from Glochidion zeylanicum var. tomentosum.

March 5, 2026

Expedient MPLC-based isolation of lupane triterpenoids from Glochidion zeylanicum var. tomentosum (Dalzell) Trimen. Bark.

Key Points

To isolate and evaluate the antimicrobial activity of lupane-type triterpenoids from Glochidion zeylanicum var. tomentosum.
Utilized automated medium-pressure liquid chromatography (MPLC) for isolation
Identified seven triterpenoid compounds
Evaluated antimicrobial activity using the minimum inhibitory concentration (MIC) method
Glochidiol showed moderate activity against S. aureus (64 µg/mL) and R. oryzae (32 µg/mL)
Compound 6 exhibited moderate antifungal activity against A. fumigatus (64 µg/mL)
Efficient MPLC isolation demonstrated the role of hydroxyl substitution in antimicrobial potency

Abstract

An automated medium-pressure liquid chromatography (MPLC) system was employed for the preparative-scale isolation of lupane-type triterpenoids from Glochidion zeylanicum var. tomentosum (Dalzell) Trimen. Seven compounds were isolated and identified as glochidone (1), glochidonol (2), glochidiol (3), lupeol (4), epilupeol (5), lup-20(29)-ene-3α,23-diol (6) and lup-20(29)-ene-1α,3β-diol (7). The antimicrobial activity of these compounds was evaluated against Micrococcus luteus, Staphylococcus aureus, Aspergillus fumigatus and Rhizopus oryzae using the minimum inhibitory concentration (MIC) method. Glochidiol (3) showed moderate activity against S. aureus (64 µg/mL) and R. oryzae (32 µg/mL). Compound 6 also showed moderate antifungal activity against A. fumigatus (64 µg/mL). The results highlight the efficiency of MPLC and the influence of hydroxyl substitution on antimicrobial activity.

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