Identification of a novel HLA-G allele, HLA-G*01:66, using PolyseqOne and oxford nanopore sequencing

Key Points

• The primary aim is to identify and characterize a novel HLA-G allele variant, specifically HLA-G*01:66.
• Utilized PolyseqOne for genomic sequencing.
• Employed Oxford Nanopore sequencing technology.
• Analyzed nucleotide substitutions in exon 3.
• Identified HLA-G*01:66 differing from HLA-G*01:01:22:01 by a single nucleotide substitution.
• The substitution occurs at position 508 in exon 3.