In this study, we established a fabrication method for three-dimensional hybrid construct by combining the cell sheet technology and the electrospinning method for polymer scaffolds. Two sheets of mouse skeletal myoblasts cells (C2C12) were prepared and laminated onto a polymeric tubular scaffold to construct a 3D hybrid structure. Mechanical tensile tests revealed that the hybrid construct exhibited enhanced Young’s modulus, tensile strength, and fracture energy compared to the pure tubular scaffold. By comparing the stress-strain curves, it was demonstrated that the deformability of tubular structure dramatically increased due to the introduction of cell sheets. These findings suggested that such biological composite structure offers improved mechanical properties suitable for cardiac tissue engineering applications. This work provides a foundational approach toward developing functional cardiac tissues for regenerative medicine.
TAJIRI et al. (Wed,) studied this question.