What question did this study set out to answer?

The study aims to investigate how hypericin and Hypericum perforatum extract affect oxidative stress factors in olfactory ensheathing cells under high glucose conditions.

April 1, 2026

Investigating the Effect of Hypericin and Hypericum perforatum L. Extract on Oxidative Stress Factors in Olfactory Ensheathing Cells of Newborn Rats Under High-Glucose Conditions

Key Points

The study aims to investigate how hypericin and Hypericum perforatum extract affect oxidative stress factors in olfactory ensheathing cells under high glucose conditions.
Extracted olfactory ensheathing cells from neonatal rat olfactory mucosa.
Cultured cells in normal and high glucose conditions to simulate diabetic neuropathy.
Isolated hypericin from Hypericum perforatum using Soxhlet extraction and quantified using HPLC.
Measured cell viability and levels of reactive oxygen species and nitric oxide after treatment.
Utilized immunocytochemistry to confirm OEC identity and ANOVA for statistical analysis.
High glucose conditions significantly decreased the survival of olfactory ensheathing cells.
Lower concentrations of hypericin and Hypericum perforatum extract increased cell survival rates.
Protective concentrations led to decreased production of reactive oxygen species and nitric oxide in high glucose conditions.

Abstract

Introduction:: High glucose status can worsen the biology of neurons; therefore, diabetic neuropathy is one of the common complications of diabetes. Olfactory Ensheathing Cells (OECs) have special properties, such as reducing inflammation. Hypericin and the Hypericum perforatum L. have various pharmacological properties, including antioxidant properties. Therefore, in this study, the effects of different concentrations of hypericin and H. perforatum extract have been investigated on oxidative stress factors in OECs by an in vitro method. Materials and Methods:: OECs were extracted from neonatal rat olfactory mucosa and cultured in normal (NG: 5.5 mM) or high glucose (HG: 30 mM) conditions. Hypericin in the H. perforatum extract was isolated via Soxhlet extraction and quantified using HPLC. Cell viability (MTT assay), Reactive Oxygen Species (ROS), and Nitric Oxide (NO) production were measured after treatment with protective (0.5 and 1 μM) and toxic (25 and 50 μM) hypericin concentrations. Immunocytochemistry confirmed OEC identity. Statistical analysis used ANOVA with (P ≤ 0.05). Results:: The results of the MTT test showed that high glucose conditions significantly decrease the survival of OECs, but using lower (protective) doses of hypericin and H. perforatum extract causes an increase in cell survival rate. Also, the protective concentrations of hypericin and hypericum extract in high glucose conditions decrease the production of Reactive Oxygen Species (ROS) and Nitric Oxide (NO) in OECs. Discussion:: Protective hypericin and H. perforatum extract concentrations mitigated HGinduced oxidative stress, aligning with prior reports of their antioxidant and anti-inflammatory effects. The dual role of hypericin and H. perforatum extract (protective at low doses, toxic at high doses) underscores their concentration-dependent therapeutic potential. These findings suggest hypericin and H. perforatum extract’s utility in diabetic neuropathy treatment when combined with OECs. Conclusion:: The results of this study showed that the protective concentrations of hypericin or hypericum extract, in high glucose conditions similar to what occurs in diabetes, positively affect reducing oxidative factors.

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