Hexamethonium and methylicaconitine enhanced the hypotensive effect of the WTX central loop fragment, while atropine weakened it, indicating both nicotinic and muscarinic receptor involvement.
Do acetylcholine receptor blockers affect the hypotensive effect of the WTX central loop fragment in rats?
Both nicotinic and muscarinic acetylcholine receptors are involved in the hemodynamic effects of the WTX central loop fragment in rats.
Absolute Event Rate: 0% vs 0%
We previously demonstrated that fragments of the central loop of the non-conventional toxin WTX reduce blood pressure in rats and inhibit certain nicotinic acetylcholine receptor subtypes 10. In the present study, we examined the effects of hexamethonium and methylicaconitine, which are nicotinic acetylcholine receptor inhibitors, as well as of atropine, a muscarinic acetylcholine receptor inhibitor, on the hypotensive effect of the WTX central loop fragment. For this purpose, these compounds were administered intravenously before the peptide. We found that hexamethonium and methylicaconitine enhanced the fragment’s hypotensive effect, while atropine weakened this effect. Only methylicaconitine enhanced the tachycardic effect of the peptide. These data indicate the involvement of both nicotinic and muscarinic acetylcholine receptors in the hemodynamic effects of the WTX central loop fragment.
Severyukhina et al. (Mon,) reported a other. Hexamethonium and methylicaconitine enhanced the hypotensive effect of the WTX central loop fragment, while atropine weakened it, indicating both nicotinic and muscarinic receptor involvement.