We investigated the effect of temperature on disk abalone Haliotis (Nordotis) discus discus hemolymph CO2 solubility coefficient (αco2) and the apparent dissociation constant of carbonic acid (pKapp). The disk abalone hemolymph was equilibrated with a standard CO2 gas mixture between 10°C and 20°C, to obtain expressions for αco2 and pKapp as a function of temperature. The relationship between αco2 and temperature (T) is expressed as follows: αco2 = 74.005 1.2936 • T 0.00944 • T2 . And the relationship between pKapp and temperature expressed as follows: pKapp = 6.4675 0.08682 • T + 0.003996 • T2 . In these equations, the parameter units are °C for T and µM/L/torr for αco2. The non-bicarbonate buffer values (ꞵNB), obtained as a regression coefficient relating pH and HCO3 – , were 2.5 Slykes at 10°C, 2.2 Slykes at 15°C, and 3.4 Slykes at 20°C. These equations enable estimation of hemolymph αco2 and pKapp between temperatures of 10°C and 20°C.
Handa et al. (Fri,) studied this question.