Abstract 4711: Investigating synergistic impact of diet-induced hyperinsulinemia and mutant Kras on colorectal cancer metastasis

Key Points

• This research aims to understand how a high-fat-sugar diet and Kras mutations interact to influence colorectal cancer metastasis.
• Mice were randomized to receive a low-fat-sugar or high-fat-sugar diet to induce hyperinsulinemia.
• Metabolic dysfunction was assessed through Echo-MRI, glucose tolerance tests, and histopathology.
• Tumor growth rates and metastasis were monitored post-tumor induction using micro-CT imaging and tissue analysis.
• In vitro studies with isogenic colorectal cancer cell lines evaluated insulin's effects on cell migration and invasion.
• HFS-fed mice showed increased body fat percentage and decreased glucose tolerance compared to LFS-fed mice.
• Higher liver disease scores and noticeable hepatocyte ballooning were observed in the high-fat diet group.
• Increased hepatic steatosis was recorded in mice consuming a 45% HFS diet, indicating metabolic dysfunction influenced by diet.