Abstract Background 25ng/ml) and interferon-gamma (IFN-γ; 25ng/ml), incubated for 24 hrs at 37°C/5%CO2, their morphological alterations, multi-lineage differentiation potentials (osteocytes/chondrocytes/adipocytes), immunophenotypying (CD90+73+105+44+34-45-19- HLADR-), Adhesion molecules (ICAM, VCAM, NCAM) migration capabilities were examined.Label-free quantitative LCMS (nLC/MS/MS coupled to Orbitrap fusion Tribrid mass-spectrometer) analysis was performed on more than 2400 proteins identified. Further, immunogenic potentials are being tested using PBMNCs from healthy human donors. Result: hWJMSCs primed with TNF-α (hWJMSC-T)/IFN-γ (HWJMSC-I) showed 90% expression of CD90+73+105+44+. Microscopically morphological distinction stained with phyalloidin/DAPI observed showed hWJMSC-T as elongated and flattened cells and Complement Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 5186.
Choudhury et al. (Fri,) studied this question.