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A number of high molecular weight compounds have been separated as constituents of normal urine. Regardless of the investigators' intention, in practically all of these studies only one or a few components were isolated. Interpretation and cor- relation of these numerous data, obtained by vari- ous procedures, would be greatly facilitated by the quantitative determination of the amount and composition of the total biocolloid content of nor- mal 24 hour urine specimens. Hamerman, Hatch, Reife, and Bartz (1) have reported the recovery of the total nondialyzable solids (TNDS) from three 24 hour urine specimens by alternate dialysis and vacuum distillation with final lyophilization.
King et al. (Sat,) studied this question.