Donepezil hydrochloride (DPL) serves as an essential pharmacological intervention for addressing cognitive deterioration linked to Alzheimer's disease (AD) and various other forms of dementia, necessitating meticulous dose regulation to attain optimal therapeutic efficacy while safeguarding patient welfare. This investigation, employing silver nanoprisms (AgNPrs) as a novel optical sensing element, seeks to establish a parafilm-integrated framework for the optical identification of DPL in phosphate buffer at pH = 9. The measurement of DPL concentrations was conducted over a linear range from 0.025 to 40 μM. The electrostatic engagement between DPL and AgNPrs was substantiated through zeta potential (Z p ) evaluation subsequent to the integration of the optical probe with the target analyte. The interaction between AgNPrs, functioning as the optical nanoprobe, and DPL, as the analyte, was validated by the alteration in Z p from −13.6 to −4.99 mV. Furthermore, the quantification of DPL in authentic samples was executed utilizing digital image analysis facilitated by smartphone technology. Subsequently, two pioneering microarrays were constructed utilizing Parafilm and poly (methyl methacrylate) (PMMA) as appropriate substrates, which facilitated single-drop colorimetric chemical assays of DPL by capturing images with a smartphone camera and subsequently analyzing these images through an Instant-eyedropper application installed on Windows. For the inaugural time, two innovative micro-scale portable colorimetric substrates (μPCSs) have been introduced, enabling the economical, expeditious, and on-site evaluation of DPL (as the model analyte) with a low limit of quantification of 0.025 μM in standard and human plasma samples. This research accentuates the cost-effectiveness of DPL detection by presenting a streamlined optical testing approach, thus providing substantial advantages over conventional analytical methodologies. This study unveils two novel μPCSs predicated on Parafilm and PMMA, facilitating smartphone-assisted in-situ detection of DPL in blood plasma samples.
Behyar et al. (Wed,) studied this question.