In vitro evaluation of gamma irradiation versus autoclaving on the morphology and regenerative potential of Allo-demineralized dentin matrix

Key Points

• The study aims to compare the effects of gamma irradiation and autoclaving on the characteristics of Allo-DM scaffolds.
• Conducted in vitro evaluations of Allo-DM scaffolds
• Compared gamma irradiation and autoclaving methods
• Assessed structural, chemical, and biological characteristics
• Measured cell survival, metabolic activity, and alkaline phosphatase activity
• Gamma irradiation preserved structural integrity better than autoclaving
• Gamma-treated scaffolds showed higher cell survival rates
• Increased metabolic activity was observed in gamma-irradiated scaffolds
• ALP activity was significantly greater in gamma-treated scaffolds compared to autoclaved ones