Background: Synthetic intraoral topical anesthetics, such as lignocaine and benzocaine can cause adverse effects in pediatric dentistry, creating a need for safer plant-based alternatives.Anacyclus pyrethrum (A.pyrethrum) and Commiphora myrrha (C.myrrha) have traditionally demonstrated anesthetic and analgesic properties.Therefore, we aimed to evaluate the phytochemical composition, cytotoxicity, and molecular docking interactions of A. pyrethrum and C. myrrha extracts for potential use as novel herbal topical anesthetic gels.Methods: Ethanolic extracts of A. pyrethrum roots and C. myrrha resin were prepared by Soxhlet extraction and maceration, respectively.Phytochemical profiling was performed using High-Performance Liquid Chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and Fourier-Transform Infrared Spectroscopy (FTIR).Cytotoxicity was assessed in L929 fibroblasts and neural cells using the MTT assay.Molecular docking and codocking of pyrethrin and furanoeudesma-1,3-diene with neuronal sodium, potassium, and GABA-A receptors were performed.Results: HPLC and GC-MS confirmed the presence of bioactive compounds, including pyrethrin and furanoeudesma-1,3-diene, and the functional groups were validated by FTIR.Cytotoxicity assays revealed high biocompatibility, with half-maximal Inhibitory Concentration (IC) values of 53 0.21 g/mL for A. pyrethrum and 54 g/mL for C. myrrha.Molecular Docking studies showed strong binding affinities (-6.1 to -8.2 kcal/mol) across target receptors, and co-docking demonstrated synergistic ligand-receptor interactions.Conclusion: Phytochemical, cytotoxic, and molecular docking analyses confirmed the anesthetic potentials of A. pyrethrum and C. myrrha.These findings support their further development as safe plant-based topical anesthetic gels.
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