Modifying the Longin method with a lower reflux temperature improves collagen extraction by reducing protein degradation.
A re-evaluation of the Longin collagen-extraction method shows that a lower reflux temperature reduces degradation of protein (“collagen”) remnants. This allows additional purification through ultrafiltration to isolate the >30kDalton fraction of the reflux product.
Brown et al. (Fri,) studied this question.