Design and ICH validation of a stability-indicating green RP-HPLC method for 5,6,7-trihydroxyflavone with multi-metric greenness evaluation and application to nanopharmaceutical systems

Key Points

• This research aims to develop a stability-indicating RP-HPLC method for the analysis of baicalein, ensuring environmental sustainability.
• Developed a buffer-free RP-HPLC method for rapid analysis
• Included multi-metric greenness evaluation
• Applied method to nanopharmaceutical systems
• Successfully validated the RP-HPLC method for stability indication
• Integrated results show the method is simple and sustainable
• Demonstrated applicability to nanoformulation analysis