Population Genetic Characteristics of 27 Autosomal STRs in the Moscow Residents

In two samples representing different generations of Moscow residents, the frequencies of 27 autosomal STR loci (D1S1677, D1S1627, D2S1360, D2S1776, D3S1744, D3S4529, D3S3053, D4S2364, D4S2366, D4S2408, D5S2500, D6S474, D7S1517, D8S1132, D9S2157, D9S1122, D10S1435, D10S2325, D11S4463, D12ATA63, D14S1434, D17S974, D17S1301, D20S1082, D21S2055, Penta D, Penta E) not included in the CODIS system were investigated. The analysis was performed using a multiplex PCR assay with fluorescent primers developed by Gordiz (Moscow). In the samples under study, the frequency parameters and population genetic and identification parameters commonly used in population genetics and forensic medicine (e.g., Hobs, Hexp, PD, MP, PE, TPI) for the 27 STR loci were determined. For the samples studied, a low insignificant level of genetic differentiation was revealed: FST = 0.00082, p = 0.0527 ± 0.0064, which allowed for the combination of the samples of the residents of Moscow into one dataset. The high power of discrimination (PD) of the kit of 27 STR loci for DNA identification in the population of the megalopolis was demonstrated. The random match probability (MP) of genotypes across all 27 STR loci in the Moscow population was calculated to be 2.1507 E–30. On the basis of the data obtained, it is concluded that these 27 STR loci can be used for DNA identification in the population of the megalopolis as an adjunct to the CODIS set for kinship determination in complex cases, given their high power of discrimination and low estimate of matching probability (MP). Despite the relatively small size of the sample studied, the established profiles of allele frequencies for the 27 STR loci can serve as a foundation for creating a reference database. This database would be used for DNA identification in forensic examinations and kinship analysis for Moscow residents, provided that the sample size is increased.