When motion slows: intrinsic photophysics of thioflavin T and X cations at cryogenic temperatures

Key Points

• This research aims to explore the intrinsic photophysics of thioflavin T and X cations under cryogenic conditions.
• Utilized gas-phase spectroscopy to investigate intrinsic photophysics.
• Recorded fluorescence-excitation and emission spectra of thioflavin T and X cations.
• Analyzed data in the absence of a microenvironment like solvents or proteins.
• Identified specific characteristics of fluorescence-excitation and emission spectra.
• Demonstrated how cryogenic temperatures affect the photophysical properties of the dyes.