Yanbian cattle hemoglobin-derived peptide NFGYDL exhibited strong ACE inhibition (IC50 = 54.95 μM) and increased angiotensin II-induced nitric oxide production in HUVEC assays.
Do ACE-inhibitory peptides derived from Yanbian cattle hemoglobin exhibit antihypertensive activity in vitro?
Peptides derived from Yanbian cattle hemoglobin demonstrate significant ACE-inhibitory activity and modulate endothelial function in vitro, suggesting potential as natural antihypertensive agents.
Effect estimate: IC50 54.95 μM
The global burden of hypertension continues to rise, highlighting an urgent need for effective therapeutic strategies. Angiotensin-converting enzyme (ACE) is central to blood pressure regulation, but commonly used synthetic ACE inhibitors often have adverse side effects, spurring the search for safer natural alternatives. The aim of this study was to investigate Yanbian cattle hemoglobin as a novel precursor for ACE inhibitory peptides. The <1 kDa fraction was identified as exhibiting the highest inhibitory activity through the systematic screening of hydrolysates across multiple molecular weight ranges. LC-MS/MS analysis identified 1980 peptides, of which four were selected for further experiments. Solid-phase synthesis confirmed that NFGYDL exhibited the strongest ACE inhibition (IC50 = 54.95 μM). Inhibition kinetics showed FHDYL acted as a mixed-type inhibitor, DLGHF and NFGYDL as competitive inhibitors and GFHLD as a non-competitive inhibitor. Molecular dynamics simulations validated the stable binding of these bovine blood-derived peptides to the ACE complex. HUVEC functional assays demonstrated that four peptides significantly increased angiotensin II-induced nitric oxide production and endothelin-1 levels, suggesting their potential antihypertensive activity. These findings suggested that bovine blood is a promising natural source of ACE-inhibitory peptides and holds potential for application as a functional component in functional foods targeting hypertension management.
Yang et al. (Fri,) conducted a other in Hypertension. Yanbian cattle hemoglobin-derived peptides (e.g., NFGYDL) was evaluated on ACE inhibition (IC50 54.95 μM). Yanbian cattle hemoglobin-derived peptide NFGYDL exhibited strong ACE inhibition (IC50 = 54.95 μM) and increased angiotensin II-induced nitric oxide production in HUVEC assays.