Abstract Sarcomphalus joazeiro (Mart. ) Hauenschild, Rhamnaceae, a native Brazilian plant, has traditional use against fungal infections. In this study, we report a rapid and selective isolation of 3- O -α- l -arabinofuranosyl- (1 → 2) -β- d -glucopyranosyl- (1 → 3) --α- l -arabinopyranosyl jujubogenin (bacopaside X) from the S. joazeiro hydroethanolic leaf extract using high-speed countercurrent chromatography (HSCCC) and reversed-phase liquid chromatography (RP-LC). After screening 11 biphasic solvent systems, the ethyl acetate: n -butanol: water system (1: 0. 4: 1, v/v/v), operated under pH-zone refining conditions in reversed-phase mode, provided optimal stationary phase retention (81%) and allowed the isolation of bacopaside X. The structure was confirmed by 1 H and 13 C-NMR. Bacopaside X exhibited potent antifungal activity against Candida spp. , with MICs between 156 and 625 µg/ml, up to 20-fold more active than the crude extract or n -butanolic fraction. This is the first report of targeted HSCCC isolation of bacopaside X from S. joazeiro, highlighting HSCCC’s value in isolating and purifying compounds from complex mixtures. Graphical Abstract
Neto et al. (Sun,) studied this question.