As global food security challenges intensify, edible crickets are recognized as sustainable protein alternatives; however, genomic resources for commercially important species remain limited, restricting evolutionary inference and the development of robust tools for farm management. We sequenced and assembled the complete mitochondrial genomes of Gryllus bimaculatus and provided the first report for Teleogryllus mitratus, both derived from commercial farms in Thailand, using high-throughput Illumina sequencing, achieving high coverage depths of 32,391× and 63,258×, respectively. The circular mitochondrial genomes were 15,955 bp and 16,046 bp and exhibited the typical insect mitochondrial gene complement of 37 genes, with a strong AT bias. Selective pressure analyses indicated pervasive purifying selection across all protein-coding genes (PCGs) (ω < 1), while episodic diversifying selection was detected in cox1, cox3, cytb, and nad5; additionally, atp8 displayed a comparatively elevated ω. Codon usage analyses revealed a strong preference for AT-ending codons, with leucine codons showing the highest bias. Phylogenetic analyses using concatenated protein-coding and ribosomal RNA genes recovered well-supported relationships within Gryllidae. These farm-derived mitogenomes provide practical foundations for molecular species authentication, population monitoring, and comparative analyses relevant to breeding and traceability. Furthermore, they provide candidate loci for future investigations into mitochondrial evolutionary dynamics and the potential development of molecular markers for commercial breeding management.
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