Intron editing of RISBZ1 confers thermotolerance for grain filling in rice

Key Points

• To investigate the effects of intron editing of RISBZ1 on grain filling and thermotolerance in rice.
• Performed intron editing on RISBZ1 to eliminate alternatively spliced transcripts.
• Analyzed grain weight and quality under normal and heat stress conditions.
• Evaluated key agronomic traits post-editing.
• Intron editing significantly increased grain weight under heat stress conditions.
• No compromise on key agronomic traits was observed under normal conditions.
• Grain quality was enhanced alongside increased weight under heat stress.