One-pot expression of an oligoclonal nanobody-based antivenom in growth-decoupled Escherichia coli.

Key Points

• To investigate the efficient production of oligoclonal nanobody-based antivenoms in a specific bacterial host.
• Utilized growth-decoupled Escherichia coli for nanobody expression
• Focused on one-pot production system for multivalent therapeutics
• Assessed broad neutralization capabilities of the recombinant antivenoms
• Successfully produced oligoclonal nanobodies with high yield
• Demonstrated effective neutralization against a variety of antigens
• Indicated potential for rapid and scalable antivenom production