ABSTRACT Enhancing the bioavailability of B vitamins can increase their biological efficacy while reducing resource use in supplement production. Bioavailability can be improved through strategies such as microencapsulation, micellization, or the application of plant‐derived bioenhancers. However, the influence of these approaches on B vitamin absorption remains largely unclear. In this study, we investigated the effects of various bioenhancers, including ginger, turmeric, black pepper, and spearmint, in both non‐micellized and micellized formulations on vitamin B uptake using a Caco‐2 enterocyte cell model. Our results indicated that tailored combinations of bioenhancers, particularly in micellized form, increased the cellular uptake of vitamins B1, B5, B6, and B7. Analysis of vitamin B transporter gene expression revealed no direct transcriptional effects of the bioenhancer formulations. Untargeted metabolomics revealed bioenhancer‐dependent alterations in lipid and amino acid metabolism, suggesting that cellular adaptations are linked to membrane organization. Finally, single‐molecule fluorescence microscopy demonstrated that bioenhancer application increased the diffusion of a membrane‐anchored model protein, suggesting that changes in membrane fluidity may contribute to the improved vitamin B uptake observed. These findings provide new insight into the cellular mechanisms underlying the increase in vitamin bioavailability and highlight the potential application of natural bioenhancers in optimized vitamin formulations.
Preinfalk et al. (Sun,) studied this question.