Abstract Background: Prostate cancer (PCa) is one of the main causes of death in men across the world. Particular focus has been placed on its preventative and treatment techniques, which include focusing on the activity of 5-alpha reductase enzyme which is responsible for the conversion of testosterone to dihydrotestosterone. These androgens are essential for prostatic development and function as well as for cells’ proliferation and survival. Objective: The aim of this study was to evaluate the anti-proliferative, antioxidant, and anti-inflammatory effects of dutasteride alone or in combination with doxorubicin on the androgen-sensitive prostate cancer cell line LNCap. Materials and Methods: LNCap cells were incubated with dutasteride alone or combination of dutasteride with doxorubicin. MTT 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay, caspase 3, tumor necrosis factor (TNF)-alpha, and total anti-oxidant capacity were assessed after 48 h to evaluate anti-proliferative, apoptotic, anti-inflammatory, and anti-oxidant effects of dutasteride. Results: Dutasteride alone decreased cells viability and TNF-alpha and increased caspase 3 and total anti-oxidant capacity in LNCap cells. The combination of dutasteride with doxorubicin showed synergistic effects on cell viability, caspase 3, and TNF-alpha with no significant effects on total antioxidant capacity in LNCap cells. Conclusion: Dutasteride alone has anti-proliferative effects with apoptotic, anti-inflammatory and anti-oxidant effects on LNCap cells but when combined with doxorubicin the anti-oxidant effects of dutasteride decreased and other properties increased.
Jihad et al. (Thu,) studied this question.