An Adenine-Based Molecular Rotor as a Fluorescent Nucleobase with High Brightness

Key Points

• This research focuses on developing a new fluorescent nucleobase based on adenine to enhance the brightness and utility of nucleic acids in various applications.
• Chemical modification of adenine to create a fluorescent molecular rotor,
• Evaluation of brightness and stability in nucleic acid frameworks,
• Applications tested in PCR and sequencing.
• Achieved significantly higher brightness compared to standard nucleobases,
• Improved performance in PCR and sequencing applications,
• Demonstrated potential for nanodevice design and aptamer technology.