3D and 4D printing hydroxyapatite-based scaffolds for bone tissue engineering and regeneration

The osseous tissue can be classified as a nanocomposite that encompasses a complex interweaving of organic and inorganic matrices. This intricate amalgamation consists of a collagen component and a mineral phase that are intricately arranged to form elaborate and perforated configurations. Hydroxyapatite, whether synthesized artificially or obtained from natural sources, has garnered considerable attention as a composite material in the field of bone tissue engineering due to its striking resemblance to bone in terms of structure and characteristics. Hydroxyapatite (HA) constitutes the predominant ceramic biomaterial for biomedical applications due to its ability to replicate the mineral composition of vertebrate bone. Nonetheless, it is noteworthy that the present biomimetic substance exhibits unfavorable mechanical characteristics, characterized by insufficient tensile and compressive strength, thus rendering it unsuitable for effective employment in the field of bone tissue engineering. Due to its beneficial attributes, hydroxyapatite (HA) is frequently employed in conjunction with various polymers and crosslinkers as composites to enhance mechanical properties and overall efficacy of implantable biomaterials engineered. The restoration of skeletal defects through the use of customized replacements is an effective way to replace damaged or lost bone structures. This method not only restores the bones' original functions but also reinstates their initial aesthetic appearance. The utilization of hydroxyapatite-polymer composites within 3D-printed grafts necessitates meticulous optimization of both mechanical and biological properties, in order to ensure their suitability for employment in medical devices. The utilization of 3D-printing technology represents an innovative approach in the manufacturing of HA-based scaffolds, which offers advantageous prospects for personalized bone regeneration. The expeditious prototyping method, with emphasis on the application of 3D printing, presents a viable approach in the development of bespoke prosthetic implants, grounded on healthcare data sets. 4D printing approach is an evolved form of 3D printing that utilizes programmable materials capable of altering the intended shape of printed structures, contingent upon single or dual stimulating factors. These factors include aspects such as pH level, temperature, humidity, crosslinking degree, and leaching factors.