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Abstract Glucosinolates were hydrolysed with endogenous myrosinase to liberate glucose by ball‐milling whole seed with water. Similar ball‐milling with acidified 40% methanol deactivated myrosinase and allowed free glucose in the seed to be measured separately. Extracts were filtered through activated charcoal paper to remove interfering phenolic compounds, and glucose was measured by a glucose oxidase/peroxidase colorimetric procedure. Correction was made for free glucose before calculating total glucosinolate content of the seed. The procedure was simple, reproducible and rapid (30 min) and the addition of exogenous myrosinase was unnecessary. It gave figures in excellent agreement with the more lengthy Sephadex micro‐column method and the proposed EEC reference procedure based on temperature‐programmed gas‐liquid chromatography of trimethylsilyl derivatives formed from enzymatically produced desulphoglucosinolates.
Smith et al. (Thu,) studied this question.