What does this research mean for the field?

A novel technique utilizing a rolling-circle substrate attached to a TIRF microscope-mounted flow chamber enables the real-time visualization and precise measurement of single-molecule DNA replication kinetics and processivities. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

January 20, 2009Open Access

Real-time single-molecule observation of rolling-circle DNA replication

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Abstract

We present a simple technique for visualizing replication of individual DNA molecules in real time. By attaching a rolling-circle substrate to a TIRF microscope-mounted flow chamber, we are able to monitor the progression of single-DNA synthesis events and accurately measure rates and processivities of single T7 and Escherichia coli replisomes as they replicate DNA. This method allows for rapid and precise characterization of the kinetics of DNA synthesis and the effects of replication inhibitors.

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Tanner et al. (Tue,) studied this question.

synapsesocial.com/papers/6a04fcda3793715fc8a098d9 https://doi.org/https://doi.org/10.1093/nar/gkp006

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