Assessment of Protein Conformation via Diazirine-Promoted Oxidation of Methionine and Tryptophan Residues

Key Points

• The research aims to evaluate how diazirine-promoted oxidation can distinguish between different protein conformations.
• Assessed holo- and apo-myoglobin alongside native and copper-bound β-2-microglobulin.
• Applied diazirine-promoted oxidation to evaluate changes in protein structure.
• Diazirine oxidation effectively differentiated protein conformations in all tested systems except ubiquitin.