Real-time Investigating Two Conformational Changes of an Adenosine Aptamer with Single-Molecule Fingerprint Signals

Key Points

• This research aims to explore the real-time conformational changes of adenosine/aptamer complexes.
• Utilized fingerprint signals for detection
• Analyzed two conformations corresponding to kinetic and thermodynamic products
• Implemented multi-parameter fingerprint-based discrimination
• Identified two distinct conformational states of the adenosine/aptamer complex
• Demonstrated the ability to monitor conformational changes in real-time
• Showed differentiation based on kinetic versus thermodynamic characteristics