Multistage Responsive NanoCRISPR Unleashes Cascade Amplified Endogenous Apoptosis and Inhibits Epithelial-Mesenchymal Transition Simultaneously for Suppressing Tumor Growth and Metastasis

Key Points

• The aim is to evaluate a novel NanoCRISPR system that induces cell death and inhibits tumor progression.
• Utilized a nanoscale CRISPR technology targeting survivin to induce apoptosis and inhibit epithelial-mesenchymal transition (EMT).
• Tested in tumor cell lines with assessments on subcutaneous tumor growth and peritoneal metastasis.
• Achieved significant suppression of subcutaneous tumor growth with minimal side effects.
• Successfully inhibited metastasis to the peritoneum through combined mechanisms of apoptosis induction and EMT suppression.