Bacterial Efficacy of Ca(oH) 2 Against E.faecalis Compared with three Dental Lasers on Root Canal Dentin- An Invitro Study

AIM: The aim of this study was to evaluate bactericidal effect of Ca(OH)2 compared with 810 nm diode, 980 nm diode, and Nd:YAG lasers on root canal dentin against E.faecalis. MATERIALS AND METHODS: Sixty five freshly extracted human mandibular single rooted teeth were selected for the study. The apical third of these roots was gradually enlarged until reaching the ISO 40- K file. The samples were divided into 4 groups, each containing 15 teeth and 5 teeth for control group. Group-1: 810 nm Diode; Group-2: 980 nm Diode; Group-3: Nd:YAG; Group-4: Ca(OH)2. 50μL of the E.faecalis ATCC 29212 strand was incubated in 1 mL of Brain Heart Infusion Broth (BHI) culture medium in37(o)C incubator for 4h. The concentration of the inoculation was then adjusted for a degree of turbidity which was adjusted to 0.5 McFarland scale. Later from the incubated broth, 10μL of E.faecalis culture was inoculated into the main canal and were sealed. RESULTS: The incubated plates were checked for growth and the colony was counted using colony counter and the results are interpreted. There was statistically significant difference (p<0.05) amongst the Ca(OH)2 group regarding the laser groups. CONCLUSION: The teeth irradiated with the Nd:YAG laser had significantly higher bacterial reduction than all the other groups and the respective control groups.