Kir4.1/Kir5.1 of distal convoluted tubule is required for short-term angiotensin-II-induced stimulation of Na-Cl cotransporter

We investigated the role of Kir4.1/Kir5.1 in mediating the effect of short-term Ang-II on Na-Cl cotransporter (NCC) expression/activity. We demonstrated that Kir4.1/Kir5.1 in the distal convoluted tubule is required for short-term Ang-II-induced stimulation of with-no-lysine-kinase 4 (WNK4), ste20-proline-alanine-rich kinase (SPAK), and NCC. However, sustained Ang-II stimulation is expected to activate WNK4, SPAK, and NCC by Kir4.1/Kir5.1-independent mechanism.