September 1, 1968Open Access

Steps in the Formation of Active Derivatives of Staphylococcal Nuclease during Trypsin Digestion

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Abstract

A staphylococcal nuclease (strain Foggi), very similar in chemical structure to the enzyme from strain V8, is cleaved in a limited way by trypsin in the presence of deoxythymidined/,5'-diphosphate and Ca++.Such treatment yields fragments PI (residues 1 to 5), PZ (residues 6 to 48), P3 (Psa, residues 49 to 149; and P3b, 50 to 149), and free lysine (residue 49).None of the products shows enzymatic activity.However, Fragments Pf and PB (either Paa or P pdTp, deoxythymidine-3',5'-diphosphate.4778

Steps in the Formation of Active Derivatives of Staphylococcal Nuclease during Trypsin Digestion

Key Points

Abstract

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