G-CSF plus DPP-IV inhibition combined with cardiomyocyte cell cycle activation significantly improved myocardial regeneration and functional recovery after myocardial infarction in mice.
Does G-CSF plus DPP-IV inhibition improve myocardial regeneration in mice expressing cyclin D2 after myocardial infarction?
Combining stem cell mobilization and homing with cardiomyocyte cell cycle activation additively enhances myocardial regeneration and functional recovery post-myocardial infarction in a mouse model.
Absolute Event Rate: 0% vs 0%
AIMS: Although pharmacological interventions that mobilize stem cells and enhance their homing to damaged tissue can limit adverse post-myocardial infarction (MI) remodelling, cardiomyocyte renewal with this approach is limited. While experimental cell cycle induction can promote cardiomyocyte renewal following MI, this process must compete with the more rapid processes of scar formation and adverse remodelling. The current study tested the hypothesis that the combination of enhanced stem cell mobilization/homing and cardiomyocyte cell cycle induction would result in increased myocardial renewal in injured hearts. METHODS AND RESULTS: Myocardial infarction was induced by coronary artery ligation in adult MHC-cycD2 transgenic mice (which exhibit constitutive cardiomyocyte cell cycle activity) and their non-transgenic littermates. Mice were then treated with saline or with granulocyte colony-stimulating factor (G-CSF) plus the dipeptidylpeptidase-IV (DPP-IV) inhibitor Diprotin A (DipA) for 7 days. Infarct thickness and cardiomyocyte number/infarct/section were significantly improved in MHC-cycD2 mice with G-CSF plus DipA treatment when compared with MHC-cycD2 transgene expression or G-CSF plus DipA treatment alone. Echocardiographic analyses revealed that stem cell mobilization/homing and cardiomyocyte cell cycle activation had an additive effect on functional recovery. CONCLUSION: These data strongly suggest that G-CSF plus DPP-IV inhibition, combined with cardiomyocyte cell cycle activation, leads to enhanced myocardial regeneration following MI. The data are also consistent with the notion that altering adverse post-injury remodelling renders the myocardium more permissive for cardiomyocyte repopulation.
Zaruba et al. (Wed,) reported a other. G-CSF plus DPP-IV inhibition combined with cardiomyocyte cell cycle activation significantly improved myocardial regeneration and functional recovery after myocardial infarction in mice.