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The rates of rat liver mitochondrial membrane-linked reactions were studied as a function of temperature, with the results being expressed as Arrhenius plots. Energy-dependent reactions involving the adenine nucleotide translocase yielded two distinct discontinuities in their Arrhenius plots, near 17.5° and 27.5°. These reactions include ADP phosphorylation, state 3 and state 4 respiration, uncoupler-stimulated respiration, dinitrophenol-stimulated adenosine triphosphatase and ATP-supported calcium uptake. Energy-dependent reactions not involving the adenine nucleotide translocase also gave two breaks in their Arrhenius plots. The upper break was near 27.5° as before. However, the temperature at which the lower break occurred was decreased to around 12.5°. These reactions included respiration-dependent calcium uptake, valinomycin-induced potassium uptake, and phosphate-induced swelling. Studies with representative matrix, outer membrane, and inner membrane enzymes including the ATPase in sonicated mitochondrial particles indicated that an intact, energy-transducing membrane is required for discontinuities in the Arrhenius plots to appear at any temperature.
Lee et al. (Sun,) studied this question.
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