Combining sequence-specific RNA cleavage by CRISPR ribonucleases with programmable RNA repair enables precise deletions and insertions for the facile engineering of RNA viruses.
This study establishes a novel recombinant RNA technology using CRISPR for precise editing and engineering of RNA viruses.
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CRISPR RNA-guided endonucleases have enabled precise editing of DNA. However, options for editing RNA remain limited. Here, we combine sequence-specific RNA cleavage by CRISPR ribonucleases with programmable RNA repair to make precise deletions and insertions in RNA. This work establishes a recombinant RNA technology with immediate applications for the facile engineering of RNA viruses.
Nemudryi et al. (Wed,) reported a other. Combining sequence-specific RNA cleavage by CRISPR ribonucleases with programmable RNA repair enables precise deletions and insertions for the facile engineering of RNA viruses.