Transcoronary arterial gene transfer of the secreted form of FGF-2 significantly improved left ventricular fractional shortening to 41% compared to 31% with saline at 14 days in microembolized rabbit hearts.
Does transcoronary gene transfer of FGF-2 improve LV systolic function and collateral vessel formation in microembolized rabbit hearts?
Transcoronary gene transfer of FGF-2 via adenovirus vector significantly improved LV systolic function and promoted collateral vessel formation in a rabbit model of coronary microembolization.
Absolute Event Rate: 41% vs 31%
p-value: p=<0.01
The effects of gene transfer of the secreted form of fibroblast growth factor-2 (FGF-2) were tested using an adenovirus vector in the microembolized rabbit heart. Japanese white rabbits underwent an intracoronary injection of 25-microm microspheres followed by recombinant adenovirus vectors encoding a secreted form of FGF-2 (FGF group), LacZ (LacZ group), or saline (saline group). Left ventricular (LV) systolic function was serially assessed by echocardiography. Vascular density was measured at 14 days with Azan and CD31 staining. The development of collateral vessels was assessed by measuring myocardial blood flow before and after the occlusion of the left anterior descending coronary artery. Percent fractional shortening (%FS) decreased after the microembolization, and improved gradually for 14 days in the FGF group only (41+/-1% (FGF) vs 32+/-1% (LacZ), 31+/-1% (saline), p<0.01). The vascular density and myocardial collateral blood flow were significantly higher in the FGF group in comparison with other groups. Transcoronary arterial gene transfer of the secreted form of FGF-2 was beneficial for the recovery of LV systolic function and development of collateral vessels in the microembolized rabbit heart.
Iwatate et al. (Mon,) conducted a other in Microembolized ischemic heart disease model (n=23). AdCAsFGF-2 (recombinant adenovirus vector encoding secreted FGF-2) vs. Saline or AdCAlacZ/Ad1w (LacZ group) was evaluated on Percent fractional shortening (%FS) at 14 days (p=<0.01). Transcoronary arterial gene transfer of the secreted form of FGF-2 significantly improved left ventricular fractional shortening to 41% compared to 31% with saline at 14 days in microembolized rabbit hearts.