The α-galactosidases cleave α-linkages of galactoside residues in galactolipids, galactomannans, glycoproteins, glycolipids, and hydrolyse α-1,6 bonds in oligosaccharides such as raffinose, melibiose, and stachyose. In this study, we evaluate the potential of non- Saccharomyces yeasts isolated from Brazilian biodiversity to produce α-gal enzyme using soy agro-industrial residue as culture medium. After screening, Torulaspora delbrueckii UFMG-CM-Y714, isolated from Bromelia karatas phytotelmo, Aureobasidium leucospermi CHAP-209, Aureobasidium sp. CHAP-213, and Aureobasidium sp. CHAP-2222, isolated from Senna macranthera flowers, were selected and grown in liquid acid protein residue of soybean (LAPRS) and tofu whey (TW). All strains grew in both residues. A. leucospermi CHAP-209 had the highest biomass production in TW (8.50 ± 0.48 g∙L -1 ), followed by Aureobasidium sp. CHAP-213 in LAPRS (8.46 ± 0.42 g∙L -1 ). The highest extracellular and intracellular specific activities were produced by A. leucospermi CHAP-209, with 0.55 ± 0.07 U∙g -1 cell mass and 69.18 ± 2.89 U∙g -1 cell mass, respectively, after 24 h of cultivation in LAPRS. Both residues were effective inducers of α-gal production. All four wild strains presented considerable biotechnological potential, with A. leucospermi CHAP-209 producing the highest enzymatic yield. Results highlight the richness of Brazilian biodiversity, which shelters new sources of diverse biocompounds, produced in inexpensive, abundant residues.
Rother et al. (Tue,) studied this question.