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Abstract A gene which specifies the activity of phosphopyruvate synthase has been located on the genome of Escherichia coli. By interrupted mating of a variety of genetic donor (Hfr) and recipient (F-) strains of Escherichia coli, K12, which carried alleles of the phosphopyruvate synthase (pps) gene, it was established that the pps gene is close to the aro-D marker. This was confirmed by phage-mediated transduction of these genes, which were shown to exhibit a high degree of linkage.
Brice et al. (Tue,) studied this question.