Human lymphoblastoid lines from lymph node and spleen

Two lymphoblastoid cell lines have been established from patients who at the time of the initiation of the cultures did not show evidence of malignant diseases. WI-L1 line originated from a lymph node biopsy of a patient who later developed a clinical course compatible with mycosis fungoides. WI-L2 line was established from the spleen of a patient having hereditary spherocytic anemia. The lines were initiated by the organ culture method and then adapted to suspension cultures. The properties of these lines are similar to those derived from Burkitt's or leukemic patients and one (WI-L1) contains a herpes-type particle.