Diagnosis of histoplasmosis by next-generation sequencing of microbial cell-free DNA in Texas

Key Points

• The aim is to evaluate the effectiveness of microbial cell-free DNA next-generation sequencing for diagnosing Histoplasma capsulatum.
• Conducted at a quaternary referral center in Texas
• Included eight patients with suspected histoplasmosis
• Assessed changes in antifungal treatment based on mcfDNA NGS results
• Five out of eight patients had a change in antifungal treatment after mcfDNA NGS results
• mcfDNA NGS provided faster turnaround times than traditional antigen tests and cultures