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A simple modified procedure is described for isolating and purifying peripherally bound membrane proteins from bovine rod outer segment disks.The methods yield milligram quantities of G-protein and cGMP phosphodiesterase which are suitable for reconstitution with membranes containing visual pigments.The properties of cGMP phosphodiesterase have been previously characterized (Baehr, W.,
Baehr et al. (Tue,) studied this question.
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