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An endonucleaseactivity present in extracts from Escherichia coli B infected with T7f phage has been purified 2500fold.This enzyme, T7 endonuclease I, is the product of gene 3, and its activity is maximal 7.5 min after infection at 37".The activity is at least 100 times greater with single stranded DNA as substrate than with duplex DNA.
Paul D. Sadowski (Fri,) studied this question.