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The effects of grouping and social rank on circulating-antibody titer were studied using C3H mice. Previously isolated mice were placed together in groups of 6 each for 4 hours per day and injected with beef serum on the fifth day of grouping. Antibody titers were determined from blood samples by the ring precipitin test. Grouped mice were found to have significantly lower titers of circulating antibody than did isolated mice. Dominant mice had significantly higher titers than the other mice in their groups. The adrenal glands of grouped mice, taken after 28 and 38 days of grouping, were significantly heavier than those of isolated mice.
Stephen H. Vessey (Wed,) studied this question.