In the presence of physiological concentrations of ATP, reduction of intracellular pH inhibits the expressed CLC-1 channel by decreasing the open probability of its common gate.
This study resolves a contradiction between native and recombinant CLC-1 channels by demonstrating that physiological ATP is required for low intracellular pH to inhibit the channel's open probability.
The CLC-1 Cl(-) channel is abundantly expressed on the plasma membrane of muscle cells, and the membrane potential of muscle cells is largely controlled by the activity of this Cl(-) channel. Previous studies showed that low intracellular pH increases the overall open probability of recombinant CLC-1 channels in various expression systems. Low intracellular pH, however, is known to inhibit the Cl(-) conductance on the native muscle membrane, contradicting the findings from the recombinant CLC-1 channels in expressed systems. Here we show that in the presence of physiological concentrations of ATP, reduction of the intracellular pH indeed inhibits the expressed CLC-1, mostly by decreasing the open probability of the common gate of the channel.
Tseng et al. (Mon,) reported a other. Low intracellular pH with physiological ATP was evaluated on CLC-1 channel open probability. In the presence of physiological concentrations of ATP, reduction of intracellular pH inhibits the expressed CLC-1 channel by decreasing the open probability of its common gate.
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